Rat sFAS/sAPO-1 (Soluble Factor-Related Apoptosis) ELISA Kit (RTES00746)

Quantitation of soluble Fas receptor in rat samples is enabled by this ELISA kit from Assay Genie, which measures sFas in serum or plasma and in tissue culture supernatant. Soluble Fas arises from alternative splicing of the Fas transcript that skips the transmembrane exon, producing a secreted decoy receptor that sequesters Fas ligand and blocks the membrane-bound death receptor pathway. Elevated circulating sFas has been reported in rat models of hepatic injury, autoimmune inflammation, and tumor progression, making the analyte a marker of interest for apoptosis-related research.

The kit ships as a complete reagent set for 96 determinations, including a pre-coated microplate, biotinylated detection antibody, recombinant standards, and substrate solution. A standard curve is generated in parallel with unknowns, and absorbance is read on a standard microplate reader. Reconstituted reagents are stored refrigerated between uses, while unused strips remain sealed in the foil pouch with desiccant to preserve antibody binding activity across the kit shelf life.