Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit

The Rat Sex Hormone Binding Globulin (SHBG) ELISA Kit from Assay Genie provides researchers with a quantitative tool to measure SHBG concentrations in rat biological samples, supporting studies in reproductive endocrinology, hepatic function, and metabolic disease. SHBG is a liver-derived glycoprotein that binds circulating androgens and estrogens, regulating the bioavailability of free sex hormones. Researchers commonly use this in vitro assay to investigate hormonal regulation in rat models of polycystic ovary syndrome, obesity, diabetes, hypothyroidism, and aging-related endocrine changes.

Each kit contains a pre-coated microplate, detection antibodies, standards, and reagents sufficient for 96 assays. The immunoassay workflow involves adding samples to wells, incubating with the conjugated detection reagent, and reading absorbance to calculate SHBG concentrations from a generated standard curve. Store kit components refrigerated between uses to preserve reagent stability, and avoid repeated freeze-thaw cycles of diluted standards.

Assay Genie provides detailed protocols including recommended dilution factors for various sample matrices, suggested sample volumes, and guidance on standard curve preparation. The kit is intended for research use only and supports applications including toxicology screening, reproductive biology research, and studies on sex hormone bioavailability in rodent models.