Rat SERPINE2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit enables quantitative measurement of SERPINE2 in rat samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. The kit employs a double-antibody sandwich format with colorimetric detection, suitable for research applications exploring the protein's role in blood clotting, fibrinolysis, inflammation, wound healing, and related physiological and pathological processes.

Performance characteristics include a sensitivity of 3.75 pg/mL and an assay range of 6.25-400 pg/mL. Linearity assessed across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions showed ranges of 83-105% for serum, 80-104% for EDTA plasma, and 83-103% for heparin plasma (n = 5). Recovery testing demonstrated average recoveries of 94% for serum (range 85-102%), 94% for EDTA plasma (range 85-101%), and 97% for heparin plasma (range 88-104%).

The workflow includes primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin conjugate binding, TMB substrate color development in the dark, and absorbance measurement at 450 nm. Components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody, HRP-Streptavidin conjugate, TMB substrate, dilution buffers, stop solution, wash buffer, plate sealers, and a technical manual. The kit is stored at 2-8°C for 12 months. SERPINE2 (Uniprot P07092) is associated with developmental biology research and is also known as GDN, GdNPF, Proteinase Nexin 1, glia-derived nexin, glial-derived neurite promoting factor, and Peptidase inhibitor 7 (PI-7).