Rat Serpin A8/Angiotensinogen ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the accurate quantitative measurement of Serpin A8/Angiotensinogen in rat samples. The assay uses a double-antibody sandwich format with colorimetric detection, and absorbance is read at 450 nm. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Performance characteristics reported by the manufacturer include a sensitivity of 0.938 ng/mL and a working range of 1.563–100 ng/mL. Linearity data show serum recovery between 90–102% (1:2), 86–104% (1:4) and 93–105% (1:8); EDTA plasma between 91–98%, 85–101% and 84–94%; and heparin plasma between 86–100%, 83–100% and 80–96%. Spiked recovery averages are 96% for serum, 93% for EDTA plasma and 99% for heparin plasma. The kit is supplied with a biotin-labelled detection antibody, HRP-Streptavidin conjugate (SABC), TMB substrate and the required buffers for use at 2–8°C.

Angiotensinogen is a key component of the renin-angiotensin system, which regulates blood pressure and fluid balance, and its dysregulation is linked to hypertension, cardiovascular disease and kidney disorders, making it a valuable biomarker for research into these conditions.