Rat Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit

Quantitative SFRP5 analysis in rat experimental models is enabled by this colorimetric immunoassay from Assay Genie, supplied as a 96 assays kit. The kit accepts serum samples, plasma samples, and tissue homogenate preparations, accommodating both systemic and tissue-level interrogation of Wnt pathway activity. SFRP5 functions as an endogenous antagonist of canonical Wnt/β-catenin signaling, and circulating or local concentrations are commonly profiled in studies of adipose tissue biology, hepatic steatosis, insulin resistance, and chronic low-grade inflammation associated with obesity and type 2 diabetes. Tumor biology investigations have additionally tracked SFRP5 expression in hepatocellular, breast, and gastric carcinoma models, where pathway reactivation through SFRP5 silencing is linked to disease progression. Each kit is delivered with a pre-coated microplate, calibrated standards, detection antibody, substrate buffer, and wash reagents needed to generate absorbance-based readouts on a standard laboratory plate reader. Researchers investigating adipokine signaling or Wnt pathway modulation in rat disease models can deploy this assay alongside complementary methods such as Western blot or quantitative PCR to triangulate transcriptional, translational, and secreted SFRP5 dynamics across treatment cohorts. The format supports single-use aliquoting of calibrators for repeated experimental runs within the kit's shelf life when stored refrigerated.