Rat Secreted Frizzled Related Protein 4 (SFRP4) ELISA Kit

Assay Genie supplies this 96-assay kit with a 12-month shelf life when stored under recommended conditions. Each lot shows intra-assay precision below 10% CV and inter-assay precision below 12% CV across low, middle, and high index concentrations during the ELISA. Spike-and-recovery validation in rat serum returned an average of 91% (range 80 to 102%), while EDTA plasma averaged 90% (range 81 to 100%) and heparin plasma averaged 84% (range 80 to 89%). Parallelism was confirmed through serial dilutions from 1:2 down to 1:16 across all three matrices. The two-antibody workflow begins with a 100 µL load of standard or sample, a 2-hour incubation at 37 °C, sequential 1-hour incubations with detection reagent A and detection reagent B at 37 °C, a 15 to 25 minute substrate development step, and a 50 µL stop solution before absorbance readout. The analyte is also known by the synonyms FRP-4 and FRPHE, and corresponds to Uniprot accession Q9JLS4. Activity loss is reported at less than 5% before the expiration date under the recommended storage regimen.