Rat SDH (Sorbitol Dehydrogenase) ELISA Kit (RTES01083)

Sorbitol dehydrogenase (SDH) is a cytoplasmic enzyme in the polyol pathway that catalyzes the NAD+-dependent conversion of sorbitol to fructose. In preclinical research, circulating SDH activity is widely used as a sensitive indicator of hepatocellular injury in rat models, supporting applications in hepatotoxicity screening, drug-safety pharmacology, and studies of polyol-pathway dysregulation in diabetes and metabolic disease.

This Assay Genie kit is built for quantitative measurement of rat SDH in liquid samples, with an absorbance-based detection scheme that runs on a standard microplate reader. The package contains reagents sufficient for 96 individual determinations, and the components are intended for research use only. Reagents should be stored at the temperatures specified on the label to maintain performance through the kit's stated shelf life, and repeated freeze-thaw cycles of prepared reagents should be avoided to preserve enzymatic reactivity.

Typical experimental contexts include monitoring liver response to xenobiotic exposure, profiling sorbitol-metabolizing enzyme activity across tissues such as liver and kidney, and correlating SORD gene expression with circulating enzyme levels. The assay workflow accommodates small sample volumes and fits naturally into multi-analyte rat biomarker panels where SDH is measured alongside ALT, AST, and LDH readouts to build a broader hepatotoxicity profile in toxicology and nutrition studies.