Rat sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit (RTES00741)

Quantitative measurement of soluble CD14 in rat samples supports studies of innate immunity, Gram-negative bacterial infection, hepatic inflammation, and metabolic dysfunction. The Assay Genie ELISA kit detects sCD14 protein shed from monocytes and hepatocytes, a circulating co-receptor that delivers lipopolysaccharide to Toll-like receptor 4 and serves as a readout of innate immune activation.

Built around a microplate immunoassay workflow, this rat-specific assay kit includes pre-coated wells, detection antibody, calibrated standards, and substrate. Bring reconstituted reagents to room temperature immediately before plate loading, and the workflow integrates with common absorbance plate readers. Intended for research use only, the kit processes serum fractions, plasma samples, and conditioned cell culture media from rat inflammation and toxicology studies.

Store kit components refrigerated between uses, avoid repeated freeze-thaw cycles of reconstituted reagents, and aliquot standards upon first thaw to preserve binding activity. Each pack contains materials sufficient for one full microplate, supporting mid-throughput investigation of CD14 pathway biomarkers across experimental cohorts.