Rat S100A8 ELISA Kit

From Assay Genie, this Sandwich ELISA kit is intended for the precise measurement of S100A8 in rat samples, supporting research into inflammatory and immune processes. S100A8 is a key protein implicated in conditions such as arthritis, inflammatory bowel disease, and cancer, and reliable quantification aids investigations of these pathological states.

The assay uses a colorimetric, double-antibody sandwich format and accepts serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. It offers a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL, with reagents stored at 2–8°C for 12 months.

Reported linearity in serum (n=5) spans 89–99%, 84–95%, and 82–99% at 1:2, 1:4, and 1:8 dilutions; EDTA plasma spans 92–105%, 87–97%, and 88–96%, and heparin plasma spans 86–100%, 83–96%, and 80–92%. Recovery averages reach 100% in serum, 99% in EDTA plasma, and 91% in heparin plasma. The kit targets rat S100A8 (UniProt P50115; Gene ID 116547; KEGG K21127), with the listed research area being cell death.