Rat S100A4 ELISA Kit

Assay Genie's ELISA kit for rat S100A4 is a sandwich-format, double-antibody immunoassay designed to detect and quantify S100A4 protein in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The colorimetric assay has a sensitivity of 4.688 pg/mL and a standard range of 7.813-500 pg/mL. S100A4 is a multi-functional protein involved in cell migration, proliferation and invasion, with overexpression linked to cancer metastasis and tumour progression; it is also implicated in inflammatory processes and tissue regeneration, making it a useful biomarker for studies of cancer biology, inflammation and tissue repair in rat models.

Performance characteristics: linearity testing (n=5) across 1:2, 1:4 and 1:8 dilutions gave recoveries of 88-101%, 85-105% and 85-101% for serum; 82-100%, 83-100% and 83-95% for EDTA plasma; and 80-97%, 81-95% and 83-95% for heparin plasma. Spike-and-recovery averages were 92% for serum (range 85-98%), 98% for EDTA plasma (range 88-104%) and 92% for heparin plasma (range 86-100%).

The procedure involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance reading at 450 nm after adding stop solution. The kit is stored at 2-8°C for 12 months.