Rat S100 Calcium Binding Protein B (S100B) ELISA Kit

Quantitative enzyme-linked immunosorbent assay for rat S100 Calcium Binding Protein B, supplied as a complete research-use kit by Assay Genie. S100B is a dimeric, EF-hand calcium-binding protein with a subunit molecular weight near 10.5 kDa, expressed predominantly in astrocytes and Schwann cells. It is established as a quantitative biomarker of astroglial activation, traumatic brain injury, neurodegeneration, and melanoma biology in rat preclinical models.

The kit supports rat-specific detection across several sample matrices commonly used in preclinical research, including tissue homogenates, cell lysates, and cell culture supernatants, in addition to liquid biological samples such as serum and cerebrospinal fluid. Components are configured for standard laboratory workflows that include incubation, washing, and a colorimetric read step. Researchers commonly pair results from this ELISA with downstream western blot or immunohistochemistry readouts to cross-validate S100B expression changes in rat tissues.

Each kit provides reagents sufficient for 96 individual assay determinations and is intended for research use only. Reconstituted standards and antibody components should be stored refrigerated and handled to limit repeated freeze-thaw cycles; the exact storage temperature and shelf life are provided in the product insert. For comparative studies, aliquoting samples prior to freezing at -20 degrees C helps preserve analyte integrity across multiple runs.

This rat S100B kit supports neuroscience, oncology, and toxicology research programs that work with rat models of central nervous system injury or peripheral nerve damage, where S100B quantification provides a quantitative readout of glial response.