Rat RUNX2/CBFA1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double antibody format for the quantitative measurement of RUNX2/CBFA1 in rat samples, including serum, plasma, tissue homogenates, cell or tissue lysates, and other liquid samples. RUNX2/CBFA1 is a key transcription factor involved in regulating bone development and mineralization, and it plays a crucial role in osteoblast differentiation and bone formation, making it a significant biomarker for studying skeletal disorders, osteoporosis, and other bone-related diseases.

The assay is colorimetric, employing a TMB substrate, and absorbance is read at 450 nm. Reported performance figures include a sensitivity of 0.188 ng/mL and a measuring range of 0.313-20 ng/mL. The kit is stored at 2-8°C for 12 months. Linearity (n=5) at 1:2, 1:4, and 1:8 dilutions is 90-103%, 87-100%, and 81-100% for serum; 85-99%, 94-100%, and 84-100% for EDTA plasma; and 85-100%, 86-96%, and 81-90% for heparin plasma. Recovery (n=5) ranges are 89-97% (average 92%) for serum, 85-101% (average 92%) for EDTA plasma, and 92-105% (average 97%) for heparin plasma.