Rat Resistin ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA (Double Antibody) kit is designed for the measurement of Resistin in rat samples. It is a reliable and accurate tool for the measurement of resistin levels in rat serum, plasma, and tissue culture supernatants, offering high sensitivity and specificity for consistent and reproducible results across a variety of research applications.

Resistin, also known by synonyms including RETN, ADSF, Adipose tissue-specific secretory factor, HXCP1, RETN1, and RSTNXCP1, is a key adipokine that plays a significant role in insulin resistance, obesity, and inflammation. By measuring resistin levels, researchers can gain valuable insights into metabolic disorders and potential therapeutic interventions. The assay is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a reported sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Components should be stored at 2-8°C for 12 months.

The protocol involves equilibration of reagents and TMB substrate, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB color development in the dark for 10-20 minutes, and stop reaction with absorbance measurement at 450 nm. Reported linearity for serum ranges from 87-104% (1:2), 82-91% (1:4) and 81-97% (1:8), while recovery averages 93% in serum across the tested range.