Rat Receptor tyrosine-protein kinase erbB-2/ErbB2 ELISA Kit

Assay Genie's sandwich ELISA, double antibody kit is designed for detecting and quantifying rat receptor tyrosine-protein kinase erbB-2/ErbB2 in rat samples. The assay accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

ERBB2, also known as HER2, is a key receptor involved in cell growth and proliferation, and its dysregulation has been linked to several types of cancer, making it a valuable biomarker for studying cancer progression and developing targeted therapies. The assay offers a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL, and components should be stored at 2-8°C for 12 months.

The workflow includes equilibrating reagents to room temperature and incubating the pre-coated plate at 37°C for 90 minutes, followed by incubation with a biotin-labeled detection antibody at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding at 37°C for 30 minutes, TMB substrate development in the dark for 10–20 minutes, and absorbance reading at 450 nm after adding stop solution.