Rat Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) ELISA Kit

Used to quantify PDK4 protein in rat specimens, the Assay Genie ELISA Kit targets a mitochondrial kinase that regulates glucose and fatty acid metabolism by phosphorylating the pyruvate dehydrogenase complex. PDK4 expression rises sharply in skeletal muscle, heart, liver, and pancreatic islets during fasting, sustained exercise, and high-fat feeding, making it a widely studied biomarker in metabolic disease research. Investigators apply this kit to monitor PDK4 protein in rat-derived specimens, accepting blood fractions such as plasma or serum alongside tissue homogenate preparations, across studies of diabetes, obesity, cardiac hypertrophy, and mitochondrial dysfunction. The assay uses a plate-based colorimetric workflow supplied as a complete kit containing pre-coated wells, lyophilized standards, biotinylated detection antibodies, streptavidin-HRP conjugate, and TMB substrate. Each well accepts a small microliter-volume sample compatible with limited rodent specimens, and the included reagents support routine use for in vitro research applications. Reagents are shipped refrigerated and stored according to the package insert to preserve antibody activity through the kit shelf life.