Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit

Pyruvate dehydrogenase kinase isozyme 1 (PDK1) phosphorylates and inactivates the pyruvate dehydrogenase complex, gating carbon entry into the TCA cycle and serving as a pivot in glucose oxidation, diabetes, cancer metabolism, and ischemia-reperfusion research. Assay Genie provides a rat-specific quantification kit for this mitochondrial regulator, accepting rat serum samples, plasma samples, and tissue homogenate specimens as input matrices. The kit ships with 96 assay wells in a colorimetric, two-antibody (sandwich) configuration that uses a standard microplate reader for absorbance quantification. Reconstituted standards and detection antibody should be kept refrigerated and shielded from light; aliquot working stocks after reconstitution to limit freeze-thaw exposure. Researchers commonly pair this kit with parallel PDH activity assays to assess kinase-mediated phosphorylation status in metabolic and cardiac studies.