Rat Pyruvate Dehydrogenase Alpha (PDHa) ELISA Kit

The PDHa ELISA from Assay Genie quantifies the alpha subunit of pyruvate dehydrogenase (PDH-E1α), the rate-limiting E1 component of the mitochondrial pyruvate dehydrogenase complex that gates pyruvate entry into the tricarboxylic acid cycle. By tracking PDHa protein abundance in rat samples, researchers can probe aerobic glucose flux, mitochondrial dysfunction, and the PDK/PDP phosphorylation axis that controls PDH activity in oxidative tissues.

Typical applications include insulin-resistant skeletal muscle, cardiac and hepatic bioenergetics, neurodegeneration models, and tumor cell metabolism, where altered PDH expression signals Warburg-type metabolic shifts. Recommended for tissue homogenates and cell lysates prepared with standard lysis buffers; pair the readout with Western blot or PDH activity assays for a full picture of complex regulation. Store reagents refrigerated at 4°C, protected from light, and avoid repeated freeze-thaw cycles of reconstituted standards to preserve calibration accuracy.