Rat PTP1B/PTPN1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double-antibody format for the quantitative measurement of protein tyrosine phosphatase 1B (PTP1B, also known as PTPN1) in rat samples. The kit is reactive with rat and is designed to accept serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported assay characteristics include a sensitivity of 0.375 ng/mL and an assay range of 0.625-40 ng/mL. Recovery for serum (n = 5) is given as 86-105% with an average of 97%, for EDTA plasma (n = 5) as 86-103% with an average of 91%, and for heparin plasma (n = 5) as 85-105% with an average of 94%. Dilution linearity (1:2, 1:4, 1:8) is reported as 85-96% / 91-103% / 89-103% for serum, 88-101% / 82-93% / 82-97% for EDTA plasma, and 85-96% / 87-97% / 84-100% for heparin plasma.

PTP1B is described as a tyrosine phosphatase involved in cell signalling through dephosphorylation of target proteins, with dysregulation linked to diabetes, obesity and cancer, making it a target of interest in therapeutic research. The kit is stored at 2-8°C for 12 months.