Rat Protoporphyrinogen Oxidase (PPOX) ELISA Kit

Built for the quantitative measurement of protoporphyrinogen oxidase (PPOX) in rat-derived samples, this colorimetric immunoassay kit from Assay Genie supports research into the heme biosynthesis pathway and porphyria-related disorders. PPOX is the penultimate mitochondrial enzyme of heme production, catalyzing the oxidation of protoporphyrinogen IX to protoporphyrin IX, and its expression is studied in rodent models of variegate porphyria and hepatic heme metabolism.

Formatted for 96 assays per kit, the assay accepts tissue homogenates from rat sources, giving laboratories a route to monitor PPOX protein levels in experimental rodent tissue. The plate-based workflow uses an absorbance readout, with all reagents supplied to complete a full microplate of standards and unknowns.

Intended for research use only, the kit fits studies of hepatic, erythroid, and neural heme pathway regulation in rats, including characterization of PPOX expression following pharmacological or genetic manipulation, and investigation of porphyrin accumulation in chemically treated rodent models.