Rat Prothyroliberin/Trh ELISA Kit

Assay Genie offers a competitive ELISA kit designed for the quantitative measurement of prothyroliberin (TRH) in rat samples. The assay uses a colorimetric detection format and is suited to serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Prothyroliberin, also known as thyrotropin-releasing hormone (TRH), is a key neuropeptide involved in regulating thyroid function and neuroendocrine pathways. The kit is intended for research applications exploring the role of TRH in thyroid dysfunction and mood disorders, among other physiological and pathological processes. It reports a sensitivity of 0.094 uIU/mL and a detection range of 0.156-10 uIU/mL, with absorbance read at 450 nm using a microplate reader following TMB-based color development.

Validation data reported by Assay Genie include linearity across 1:2, 1:4, and 1:8 dilutions for serum (89-100%, 85-102%, 88-105%), EDTA plasma (85-101%, 82-94%, 93-100%) and heparin plasma (80-93%, 82-95%, 84-98%), and recovery averages of 95% for serum, 83% for EDTA plasma, and 96% for heparin plasma. The kit should be stored sealed at 2-8°C and not cryopreserved. Synonyms for the target include Trh, Pro-TRH, and Prothyroliberin (UniProt P01150).