Rat Protein phosphatase methylesterase 1/PPME1 ELISA Kit

A competitive ELISA kit from Assay Genie is designed for the quantitative detection of Protein Phosphatase Methylesterase 1 (PPME1) in rat samples. PPME1 is an enzyme that plays a crucial role in the regulation of protein methylation and various cellular processes, and its dysregulation has been linked to cancer, neurodegenerative disorders, and metabolic disorders, making it a significant potential therapeutic target and biomarker for disease progression.

The kit uses a colorimetric, competitive ELISA format with the plate coated with antigen, and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 0.375 ng/mL and a detection range of 0.625–40 ng/mL. Storage is at 2–8°C (sealed); the reagents should not be cryopreserved.

Validated linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma samples. The procedure involves standard and biotin-antibody binding, plate washing, TMB substrate development in the dark at 37°C, addition of stop solution, and absorbance reading at 450 nm. The kit includes a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-streptavidin conjugate (SABC, 100X), TMB substrate, sample, antibody and SABC dilution buffers, stop solution, 25X wash buffer, plate sealer, and a technical manual.