Rat Protein Phosphatase 3, Regulatory Subunit 1 (PPP3R1) ELISA Kit

This ELISA kit from Assay Genie quantifies rat calcineurin B, the calcium-binding regulatory partner of calcineurin A that anchors the phosphatase to its calcium/calmodulin-dependent signaling complex. Measuring the regulatory subunit (PPP3R1) rather than the catalytic subunit provides a specific readout of calcineurin complex availability in rat specimens.

The kit is supplied in a 96-assay plate format and accepts tissue homogenates from rat samples, supporting work on isolated organs, dissected tissue, and cultured cell preparations. Calcineurin B is a ~19 kDa EF-hand protein that binds four calcium ions and forms a heterodimer with calcineurin A; tracking its abundance informs studies of T-cell activation, cardiac stress signaling, immunosuppressant drug response (cyclosporin A and tacrolimus both engage this pathway), and neural calcium signaling.

Research applications include characterization of calcineurin pathway activity in rat models of cardiovascular disease, immune regulation, neurodegeneration, and transplant rejection biology. Assay Genie provides the reagents as a complete kit, and the assay is intended for research use only.