Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit

The Assay Genie Rat Protein Kinase C Beta 1 (PKCb1) ELISA Kit enables quantitative measurement of PRKCB1, a serine/threonine kinase implicated in B-cell receptor signaling, insulin response, and apoptosis regulation. Researchers studying metabolic disease, neurobiology, and oncology where PKC beta isoforms are dysregulated rely on this kit for rat tissue homogenates and cell lysates. Each kit provides pre-coated plate strips, capture antibody, detection antibody, recombinant standard, and substrate for 96 reactions. Standard protocol requires approximately 3.5 hours from sample addition to final read, with a 100 uL sample volume per well applied in duplicate. Reagent components are stored refrigerated at 4°C and the standard should be aliquoted and frozen for repeated use beyond the first thaw cycle. This research-use-only assay complements downstream validation by western blot or immunohistochemistry, allowing researchers to correlate PKCb1 protein levels with tissue-specific localization in rodent models of diabetic complications, inflammatory responses, and diacylglycerol/phorbol ester signaling cascades.