Rat Protein Kinase, AMP Activated Beta 1 (PRKAb1) ELISA Kit

The PRKAb1 ELISA kit from Assay Genie enables researchers to quantify the AMP-activated protein kinase beta-1 subunit in rat-derived samples. AMPK beta-1 acts as a scaffold within the heterotrimeric AMPK complex (alpha-beta-gamma), anchoring the catalytic alpha subunit and the gamma regulatory subunit while also binding glycogen via its CBM (carbohydrate-binding module) domain. This subunit plays a central role in cellular energy sensing, glucose uptake, fatty acid oxidation, and mitochondrial biogenesis, making it a frequent target in metabolic disease research, exercise physiology studies, and oncology investigations.

Each kit is formatted for 96 assays and validated for use with rat tissue homogenates, cell lysates, and related sample matrices. Quantification relies on an absorbance-based measurement, providing reproducible results for comparative studies of PRKAb1 expression across experimental conditions, drug treatments, or disease models. Researchers commonly apply this tool when investigating insulin resistance, type 2 diabetes models, cardiac hypertrophy, tumor metabolism, and the molecular effects of compounds that activate or inhibit AMPK signaling pathways.

The kit from Assay Genie is supplied with the reagents needed to complete the immunoassay workflow and is intended for research use only. Standard laboratory equipment such as a microplate reader is required for signal measurement. For storage and handling, the kit components should be kept refrigerated; specific shelf-life and individual component storage conditions are detailed in the product insert accompanying each package.