Rat Prostaglandin E2/EP2 ELISA Kit

Prostaglandin E2 (EP2) acts as a key mediator of inflammation and pain, with dysregulation implicated in inflammation, cancer, and arthritis. The Assay Genie sandwich ELISA uses a double-antibody format with colorimetric detection to quantify EP2 in rat samples. Accepted matrices include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 23.438 pg/mL and the assay range is 39.063-2500 pg/mL. Unopened kits are stored at 2-8°C for 12 months.

Linearity at 1:2, 1:4, and 1:8 dilutions: serum (n=5) 92-100%, 88-104%, 89-105%; EDTA plasma (n=5) 86-99%, 82-90%, 87-100%; heparin plasma (n=5) 84-100%, 87-101%, 80-104%. Recovery ranges with averages: serum 85-103% (96%), EDTA plasma 85-102% (95%), heparin plasma 85-101% (93%). The procedure covers reagent equilibration to room temperature, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody for 60 minutes, HRP-streptavidin for 30 minutes, TMB substrate in the dark for 10–20 minutes, and absorbance at 450 nm after stop solution.

Listed synonyms are EP2, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, prostaglandin E receptor 2 subtype EP2 (53kD and 53kDa), prostaglandin E2 receptor EP2 subtype, and Prostanoid EP2 receptor. The associated UniProt entry is Q62928.