Rat Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) ELISA Kit

Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) is the catalytic alpha subunit of the collagen prolyl 4-hydroxylase tetramer, an enzyme required for proper collagen folding and stability in the endoplasmic reticulum. Quantifying P4Ha1 expression in rat tissue homogenates supports research into fibrosis, extracellular matrix remodeling, wound healing, and tumor microenvironment studies. Assay Genie supplies this microplate-format kit with capture antibody pre-coated onto a strip plate, conjugate, standards, and buffer reagents sufficient for one full plate of rat samples. Store the unopened kit at 4°C and protect from light; allow reagents to reach room temperature before running the assay. Results are read on a standard plate reader after substrate addition. The protocol is intended for research use only and is not validated for diagnostic or clinical decision-making.