Rat Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit

PIICP, the C-terminal propeptide of type II procollagen, is liberated during collagen II fibril assembly and serves as a circulating indicator of chondrocyte biosynthetic activity in articular cartilage. Quantifying this cleavage marker in rat specimens supports longitudinal monitoring of cartilage matrix turnover without requiring terminal sampling at each timepoint.

This Assay Genie kit is calibrated for rat reactivity, uses a colorimetric readout, and supplies reagents sufficient for 96 assays run on a standard microplate. Typical research applications include osteoarthritis studies, evaluation of chondroprotective or chondrodestructive interventions, growth-plate biology, and characterization of chondrogenic differentiation in vitro using cultured chondrocytes or mesenchymal stromal cells.

The workflow accommodates serum-derived or plasma-derived specimens collected from rodent bleeds, consuming only a small aliquot per well and leaving material for replicate measurements across experimental groups. Components are shipped refrigerated and protected from light; reconstituted standards and detection reagents should be stored aliquoted and frozen between uses to preserve activity across multiple freeze-thaw cycles.