Rat PRKACB/PKA beta (catalytic subunit) ELISA Kit

This Sandwich ELISA from Assay Genie is configured for the quantification of PRKACB (PKA Beta Catalytic Subunit) in rat samples, supporting research into an enzyme implicated in gene expression, metabolism, and cell growth, with dysregulation linked to cancer, diabetes, and other disorders. The double-antibody format accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types, providing a versatile tool for studies of PKA signaling and disease pathogenesis.

Performance specifications include a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Dilutional linearity across 1:2, 1:4, and 1:8 ratios (n = 5) spans 86–103% for serum, 92–98% for EDTA plasma, and 81–100% for heparin plasma. Average recovery is 98% in serum, 96% in EDTA plasma, and 100% in heparin plasma. The kit is intended for storage at 2–8°C for 12 months, and absorbance is read at 450 nm following TMB-based color development.