Rat Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

PDGFB is a homodimeric growth factor (PDGF-BB) central to mesenchymal cell recruitment, pericyte coverage of nascent vessels, fibrotic remodeling, and tumor-stroma crosstalk. This quantitative immunoassay from Assay Genie measures the PDGF-BB ligand in rat-derived samples, with intended application on cell lysates and tissue homogenates from rodent cardiovascular, renal, neural injury, and oncology research models. The kit supports reproducible longitudinal profiling of ligand abundance in experimental disease and intervention studies, including investigations of pulmonary hypertension, diabetic nephropathy, post-ischemic tissue repair, and stromal support of solid tumor xenografts.

Reagents are supplied as a complete set, including a pre-coated microplate, biotinylated detection antibody, recombinant rat PDGFB standard, streptavidin-enzyme conjugate, substrate solution, and wash concentrate, packed for cold-chain delivery. In vitro use only; intended for research applications, not for diagnostic or therapeutic decision-making. Reconstituted standards and stock detection antibody should be stored frozen in single-use aliquots to minimize freeze-thaw cycles, while working dilutions are kept refrigerated between plate-loading steps. Pairing the assay with parallel PDGFRA or PDGFRB transcript quantification can clarify whether observed ligand changes reflect autocrine, paracrine, or stromal signaling compartments within the sampled tissue.