Rat Plasminogen ELISA Kit

This Assay Genie Sandwich ELISA kit is designed for the quantitative measurement of plasminogen (Plg, Uniprot Q01177) in rat samples using a double-antibody format. The colorimetric workflow uses a biotin-labeled detection antibody and HRP-Streptavidin conjugate, with TMB substrate development and absorbance read at 450 nm. It is reactive with rat and validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 56.25 ng/mL with a working range of 93.75-6000 ng/mL. Storage is at 2-8°C for 12 months. The product falls within the cardiovascular research area, reflecting plasminogen's role in the fibrinolytic system, the breakdown of blood clots, and tissue remodeling.

Linearity ranges across 1:2 to 1:8 dilutions are 89-105% in serum, 83-96% in EDTA plasma, and 80-99% in heparin plasma. Recovery averages 97% in serum, 97% in EDTA plasma, and 92% in heparin plasma.