Rat PKLR/Pyruvate Kinase, Liver And RBC ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie is designed for the accurate quantitative measurement of rat PKLR (Pyruvate Kinase, Liver and RBC) in serum, plasma, and cell culture supernatants. The kit uses a double antibody sandwich ELISA format and provides reliable, reproducible results suited to a range of research applications.

PKLR is a key enzyme involved in glycolysis, playing a crucial role in energy metabolism and maintaining red blood cell function. Dysregulation of PKLR has been linked to conditions such as liver disease, anemia, and metabolic disorders, making it a valuable biomarker for studying these diseases and exploring potential treatments.

The assay has a sensitivity of 0.469ng/ml with a measuring range of 0.781-50ng/ml, and the kit should be stored at 4°C for 6 months. It is for Research Use Only. Performance characteristics include an intra-assay CV of less than 8% and an inter-assay CV of less than 10%. Recovery rates in spiked samples are 85-100% (average 93%) in serum, 91-101% (average 96%) in EDTA plasma, and 87-101% (average 92%) in UFH plasma. Dilutional linearity values at 1:2, 1:4, and 1:8 are reported as 88-101%/85-104%/85-102% for serum, 85-101%/85-95%/87-101% for EDTA plasma, and 82-95%/81-99%/82-96% for UFH plasma.