Rat PIINP (Procollagen II N-Terminal Propeptide) ELISA Kit (RTES01145)

Assay Genie's rat PIINP immunoassay quantifies the N-terminal propeptide of type II procollagen, a circulating biomarker that reflects synthesis of cartilage extracellular matrix. Investigators working with rodent arthritis models, growth-plate biology, and collagen-turnover disorders rely on this marker to monitor anabolic activity in articular cartilage.

Type II collagen is the principal structural protein of hyaline cartilage, and the cleaved propeptide fragment is liberated during fibril assembly, making circulating levels of PIINP an index of new collagen deposition rather than breakdown. Pairing PIINP measurements with cartilage degradation markers such as CTX-II gives a more complete picture of joint-tissue remodeling in rat models of arthritis.

Each kit ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and chromogenic substrate sufficient for 96 determinations. Reconstituted reagents should be aliquoted and stored at -20 degrees C to preserve antibody activity across repeated freeze-thaw cycles, while the working detection solution is prepared fresh before each run.