Rat Phospholipase A2, Calcium Independent (iPLA2) ELISA Kit

The Assay Genie Rat iPLA2 ELISA Kit enables quantitative measurement of calcium-independent phospholipase A2 in rat samples, supporting research into membrane lipid remodeling, ferroptosis pathways, and neuroinflammatory signaling. iPLA2 (also known as PLA2G6 or iPLA2β) hydrolyzes the sn-2 position of glycerophospholipids, releasing arachidonic acid and lysophospholipids that drive downstream inflammatory cascades.

Investigators studying Parkinson's disease models, cardiac ischemia-reperfusion injury, and hepatic lipid metabolism use this assay to track enzyme levels across individual serum samples, plasma preparations, and tissue homogenates from rat origin. The microplate-based workflow accommodates standard laboratory pipetting equipment and standard curve generation.

For best results, keep the kit refrigerated, protect components from light, and avoid repeated freeze-thaw cycles of reconstituted standards. Each kit ships with a pre-coated well plate, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution in individual vials for single-use preparation. Research use only.