Rat Phosphofructokinase, Muscle (PFKM) ELISA Kit

Phosphofructokinase, muscle type (PFKM) is the rate-limiting enzyme of glycolysis in skeletal and cardiac muscle, catalyzing the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate and subject to allosteric regulation by ATP, AMP, and fructose-2,6-bisphosphate. The Assay Genie Rat PFKM ELISA Kit delivers a quantitative immunoassay for measuring PFKM protein levels in rat tissue homogenates, supporting research in muscle metabolism, exercise physiology, and metabolic disease models such as Tarui disease (glycogen storage disease type VII), where inherited PFKM deficiency is the underlying genetic cause. The kit is supplied in a 96-assay plate format and is intended for research use only, using a colorimetric readout compatible with standard microplate readers. Common applications include investigating glycolytic flux regulation in rodent models, comparing PFKM expression across skeletal muscle and cardiac tissues, and characterizing metabolic adaptations in insulin resistance, diabetes, and cardiac hypertrophy studies.