Rat Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) ELISA Kit

This immunoassay from Assay Genie quantifies cytosolic Phosphoenolpyruvate Carboxykinase (PCK1) in rat-derived samples, supporting research in hepatic gluconeogenesis, glucose homeostasis, and metabolic-disease modeling. PCK1 catalyzes the GTP-dependent conversion of oxaloacetate to phosphoenolpyruvate, the first committed step of gluconeogenesis, and is hormonally regulated by glucagon, cortisol, and insulin, making its abundance a key readout in studies of fasting response, insulin resistance, and hepatic steatosis in rodent models.

The kit is configured for use with diverse rat sample inputs including whole-tissue lysates and circulatory fractions, and is supplied as a 96-assay pack containing all buffers, standards, and detection reagents needed to complete a full microplate run. Intended for research use only and in vitro measurement of PCK1 protein abundance rather than diagnostic application. Storage temperature conditions are specified in the enclosed insert; refrigerate short term and protect from light where indicated.