Rat Phosphodiesterase 3B, cGMP Inhibited (PDE3B) ELISA Kit

Quantitative measurement of cGMP-inhibited Phosphodiesterase 3B in rat specimens is supported by the Assay Genie in vitro ELISA Kit, formatted on a standard microplate and producing an absorbance-based readout compatible with routine cell-signaling and metabolic-disease research workflows. The kit is intended for research use only and runs as a single-use, aliquoted workflow.

Phosphodiesterase 3B hydrolyzes both cAMP and cGMP and is the cGMP-inhibited PDE isoform, making its quantitation relevant to studies of insulin signaling, cardiac contractility, adipocyte metabolism, and vascular tone. Researchers evaluating PDE3 inhibitor pharmacology, beta-adrenergic responsiveness, or rodent metabolic-disease models rely on consistent PDE3B measurements, and this plate-based assay delivers reproducible performance matched to those investigations.

Supported input matrices span a broad range of rat-derived specimen types, with the assay validated across serum-type and plasma-type fractions, tissue homogenates, cell lysates, and cell culture supernatants. This breadth allows a single kit to serve both in vivo tissue studies and in vitro cell-based experiments. Reagents are provided in aliquoted, single-use quantities sufficient to complete a full microplate run, including standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, and substrate solution.

Compatible with standard microplate readers capable of absorbance measurement, the workflow integrates into laboratories already handling routine ELISA protocols. Pre-coated strips minimize hands-on time, while the included protocol provides step-by-step guidance for sample preparation, incubation times, and curve fitting. Research applications include pharmacology screening, endocrinology investigation, and metabolic-disease studies where reliable downstream quantitation of PDE3B matters.