Rat Phosphodiesterase 3A, cGMP Inhibited (PDE3A) ELISA Kit

Phosphodiesterase 3A, cGMP-inhibited (PDE3A) is a key regulator of intracellular cyclic nucleotide signaling, hydrolyzing both cAMP and cGMP to modulate cardiovascular, metabolic, and platelet function. This quantitative immunoassay from Assay Genie is purpose-built for rat-derived matrices, enabling researchers to measure endogenous PDE3A protein levels in tissue homogenates from rat samples. Each kit contains a pre-coated microplate and the reagents needed to run the full plate, supporting studies of cardiac hypertrophy, vascular smooth muscle signaling, insulin secretion, and platelet biology where PDE3A activity is implicated. Investigators studying heart failure, diabetes, or smooth muscle disorders use this assay to track PDE3A expression changes following pharmacological inhibition or genetic manipulation. The protocol accommodates fresh or frozen tissue lysates prepared in standard lysis buffers, and the format pairs readily with downstream Western blot confirmation of expression changes, making it practical to batch process experimental cohorts alongside confirmatory blots.