Rat PGK1/Phosphoglycerate kinase 1 ELISA Kit

From Assay Genie, this sandwich ELISA kit uses a double-antibody format for the quantitative measurement of Phosphoglycerate kinase 1 (PGK1) in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The workflow involves a pre-coated microplate, a biotinylated detection antibody, HRP-conjugated Streptavidin, TMB substrate and a stop solution, with absorbance read at 450 nm.

Reported performance figures include a sensitivity of 46.875 pg/mL and an assay range of 78.125-5000 pg/mL. Recovery in serum (n=5) is given as 92-104% (average 96%), in EDTA plasma 92-100% (average 97%), and in heparin plasma 90-103% (average 95%). Components are shipped on ice packs and stored at 2-8°C for 12 months. The kit also includes a Bradford Reagent for total protein quantification to support sample normalization, and is supplied for research use only.