Rat Peroxiredoxin 1 (PRDX1) ELISA Kit

The Assay Genie Peroxiredoxin 1 (PRDX1) immunoassay kit enables quantitative measurement of endogenous PRDX1 in rat-derived research samples. PRDX1 is a ubiquitous antioxidant peroxidase that reduces hydrogen peroxide and organic hydroperoxides, and its expression is commonly tracked in rodent models of inflammation, ischemia-reperfusion injury, neurodegeneration, hepatotoxicity, and oxidative-stress-related carcinogenesis.

The kit is supplied in a microplate format with pre-coated capture antibody, biotinylated detection reagent, calibrator standards, streptavidin-enzyme conjugate, substrate, and stop solution. This layout allows researchers to run standard curves alongside experimental unknowns, vehicle-treated controls, and positive reference samples on a single plate, supporting moderate-throughput cohort comparison in a laboratory workflow.

The recommended protocol involves adding diluted rat samples and standards to the wells, washing, applying detection reagents, and reading the chromogenic endpoint at the appropriate wavelength on a standard plate reader. Concentrations are interpolated from the standard curve and reported in mass units per milliliter. Reagents are for research use only and are not validated for diagnostic or therapeutic applications. Components should be stored according to the conditions specified on the vial labels, and repeated freeze-thaw cycles of reconstituted reagents should be avoided to preserve antibody binding activity across runs.