Rat Pepsinogen C/PGC ELISA Kit

A high-quality sandwich ELISA from Assay Genie, this kit uses a double antibody format with colorimetric detection for the quantitative measurement of pepsinogen C in rat samples. The kit supports research in cell biology and the investigation of gastric health and disease.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit has a sensitivity of 2.813 ng/mL and a measurable range of 4.688-300 ng/mL. The general storage recommendation is 2-8°C for 12 months.

Pepsinogen C is a key enzyme involved in the gastric digestion process and is commonly used as a marker for gastric mucosal injury and inflammation. Monitoring pepsinogen C levels supports research into conditions such as gastritis, peptic ulcers, and gastric cancer. The assay workflow involves primary incubation at 37°C for 90 minutes, detection antibody binding at 37°C for 60 minutes, HRP-streptavidin binding at 37°C for 30 minutes, TMB substrate development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after adding stop solution.