Rat Pentosidine ELISA Kit

A competitive ELISA kit from Assay Genie, designed for the quantitative detection of pentosidine in rat samples. The kit uses a colorimetric detection method in a competitive ELISA format coated with antigen, and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Pentosidine is a key advanced glycation end product that accumulates in tissues with age and has been associated with age-related diseases such as diabetes, renal failure, and Alzheimer's disease. Measuring pentosidine in rat models allows researchers to investigate the role of glycation in these conditions and to advance work on age-related pathologies.

According to the supplied product data, the assay has a stated sensitivity of 3.75 ng/mL and a working range of 6.25–400 ng/mL. Kits should be stored sealed at 2–8 °C and must not be cryopreserved. Reported linearity across 1:2, 1:4 and 1:8 dilutions is 86–104%, 83–98% and 84–98% for serum, 85–99%, 84–101% and 80–90% for EDTA plasma, and 85–101%, 83–97% and 82–100% for heparin plasma (n = 10 each). Reported spike-recovery ranges are 85–105% (average 96%) for serum, 92–105% (average 98%) for EDTA plasma and 88–104% (average 94%) for heparin plasma.