Rat PDGFB ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of PDGFB (Platelet-Derived Growth Factor-BB) in rat samples. The assay uses a double-antibody sandwich format with colorimetric detection and a sensitivity of 18.75 pg/mL, covering a working range of 31.25-2000 pg/mL. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and reagents should be stored at 2-8°C for up to 12 months.

Reported linearity for serum (n=5) ranges from 94-105% at 1:2, 88-104% at 1:4, and 89-98% at 1:8 dilution; EDTA plasma (n=5) shows 88-105% / 84-98% / 93-100% across the same dilutions, and heparin plasma (n=5) shows 88-105% / 80-94% / 90-104%. Spike-and-recovery performance is reported as 86-105% (average 96%) in serum, 88-105% (average 95%) in EDTA plasma, and 88-104% (average 95%) in heparin plasma. PDGFB is a key growth factor involved in cell growth, proliferation, and differentiation, with roles in wound healing, embryonic development, and tissue repair, and its dysregulation is associated with fibrotic diseases, certain cancers, and vascular disorders.