Rat Patatin Like Phospholipase Domain Containing Protein 3 (PNPLA3) ELISA Kit

The Assay Genie PNPLA3 immunoassay provides researchers studying hepatic lipid metabolism with quantitative measurement of rat patatin-like phospholipase domain-containing protein 3 across tissue homogenates and related biological matrices. PNPLA3, also called adiponutrin, participates in triacylglycerol hydrolysis and lipid droplet remodeling on hepatic and adipose membranes. The I148M sequence variant of the human ortholog has been linked to non-alcoholic fatty liver disease progression and increased hepatic fat accumulation, making rat models valuable for translational metabolic research.

This in vitro kit is intended for research use only and supports investigations into adiponutrin expression across liver, adipose, and metabolic disease models. Reagents should be stored refrigerated between uses, and prepared aliquots of standard and sample should be handled according to the included protocol to preserve binding activity across repeated freeze-thaw cycles. The kit format allows batch processing of experimental cohorts, and the assay procedure follows standard curve-based quantification for typical laboratory workflows.

Components are supplied in individual vials with break-apart microplates to minimize waste when only partial runs are required. Each package includes a detailed protocol insert, plate sealers, and dilution buffers. Researchers examining PNPLA3 in relation to steatosis, insulin resistance, or hepatic fibrosis will find this tool useful for protein-level quantification that complements gene expression studies of the rat PNPLA3 locus.