Rat PACAP-38/Pituitary Adenylate Cyclase Activating Polypeptide 38 ELISA Kit

From Assay Genie, this sandwich ELISA kit is designed for the accurate quantification of PACAP-38 (Pituitary Adenylate Cyclase-Activating Polypeptide-38) levels in rat samples. PACAP-38 is a neuropeptide that plays a crucial role in regulating neurotransmission, hormone secretion and cell growth, and its dysregulation has been linked to migraine, neurodegenerative diseases and stress-related disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

Using a double-antibody sandwich format with colorimetric detection, the kit offers a sensitivity of 75 pg/mL and a working range of 125–8000 pg/mL. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with kit components stored at 2–8°C for 12 months.

Reported linearity (n = 5) for serum is 86–98% at 1:2, 90–94% at 1:4 and 86–91% at 1:8; EDTA plasma 89–98%, 83–93% and 82–93%; and heparin plasma 81–95%, 81–93% and 85–97%. Corresponding recovery averages are 92% for serum, 97% for EDTA plasma and 93% for heparin plasma.