Rat Orexin (Hcrt) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise measurement of orexin (HCRT) levels in rat biological samples. Orexin, also known as hypocretin, is a neuropeptide that plays a crucial role in regulating sleep-wake cycles, appetite, and energy metabolism, with dysregulation implicated in neurological conditions including narcolepsy and insomnia.

The assay shows a detection range of 7.8-500pg/mL and a sensitivity of 3.9pg/mL, with a reported intra-assay CV of 5.3% and inter-assay CV of 10.1%. Specificity is stated for natural and recombinant rat Orexin. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and the Research Area is listed as Metabolism. The detection method is colorimetric.

Linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma across serial dilutions. The kit is for research use only, and storage conditions vary by component, with most reagents stored at -20°C and the wash buffer, substrate, and stop solution stored at 4°C.