Rat Oncomodulin ELISA Kit

From Assay Genie, this sandwich-format immunoassay is designed for the quantitative measurement of oncomodulin in rat samples. Using a double-antibody configuration with a colorimetric readout measured at 450 nm, the assay delivers a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL, supporting detection in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Validated performance data demonstrate linearity across dilution series (1:2 to 1:8) in serum, EDTA plasma, and heparin plasma, with recovery ranges of 85-102% (serum and EDTA plasma, average 94%) and 87-105% (heparin plasma, average 95%). The workflow involves equilibrating reagents to room temperature, loading standards and samples onto the pre-coated plate, sequential incubations with a biotin-labeled detection antibody and HRP-streptavidin conjugate at 37°C, TMB substrate development in the dark for 10–20 minutes, and a stop reaction with absorbance measurement at 450 nm. Kit components are stable for 12 months at 2-8°C. Note: the printed protocol is a sample protocol; users should follow the protocol supplied with their specific kit batch.

Oncomodulin is described as a key regulator in the growth and development of tumors, making this kit a relevant tool for research on cancer progression and potential therapeutic interventions in rats.