Rat Nucleobindin 2 (NUCB2) ELISA Kit

Nucleobindin 2 (NUCB2) is a calcium-binding EF-hand precursor protein expressed in rat hypothalamus, pancreatic islets, gastric mucosa, and adipose tissue. Proteolytic cleavage of NUCB2 yields nesfatin-1, an anorexigenic peptide implicated in feeding behaviour, glucose homeostasis, and energy balance. The Assay Genie Rat NUCB2 ELISA Kit delivers quantitative measurement of this precursor in rat research specimens, supporting laboratories working on metabolic disease, obesity models, type 2 diabetes pathophysiology, and neuroendocrine signalling research.

Each kit supplies a pre-coated microplate together with calibrated standards, detection antibody, and substrate solution required to construct a standard curve and quantify unknowns via absorbance-based readout. The protocol is validated for rat serum, tissue homogenates, and cell culture supernatant, with each well receiving a small sample volume during pipetting. The pack-of-96-assay configuration supports medium-throughput screening of experimental cohorts, and all reagents are intended for research use only in vitro.

Storage and handling guidance recommends keeping reconstituted components refrigerated to preserve antibody binding activity through the stated shelf life, with repeated freeze-thaw cycles of prepared reagents not advised. Researchers in endocrinology, metabolism, and neuroscience laboratories rely on the Assay Genie kit for reproducible quantification of nucleobindin 2 in experimental rat models.