Rat Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit

Quantitative measurement of rat Nuclear Receptor Coactivator 3 (NCOA3) in tissue homogenates is enabled by this Assay Genie ELISA Kit. The target belongs to the p160/SRC family of transcriptional coactivators that interact with estrogen receptor, androgen receptor, progesterone receptor, and other ligand-activated nuclear receptors to amplify hormone-driven gene expression. Aberrant NCOA3 signaling is widely investigated in hormone-dependent tumor models, metabolic dysfunction, and reproductive endocrinology research.

Tissue homogenates are loaded alongside a serial dilution of the supplied standard to interpolate unknown concentrations via the assay standard curve. Each kit ships as a complete package containing a pre-coated plate, detection reagent, standard, and substrate in quantities sufficient for 96 individual determinations. Reconstituted components should be refrigerated and protected from light for short-term stability; aliquots can be frozen for extended storage.

The kit is configured for rat-specific detection and supports parallel measurement of NCOA1/SRC-1 and NCOA2/SRC-2 within the p160 coactivator family. It is intended for research use only and serves in vitro investigation of nuclear receptor coactivation mechanisms across rat model systems.