Rat Nuclear Factor Kappa B/NFκB ELISA Kit

A reliable colorimetric sandwich ELISA from Assay Genie for the quantitative detection of Nuclear Factor Kappa B (NF-κB) in rat samples. NF-κB is a key transcription factor that plays a critical role in regulating immune responses, inflammation, and cell survival, and its dysregulation has been implicated in cancer, autoimmune disorders, and inflammatory conditions. The kit is suitable for use with rat serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples.

It uses a sandwich ELISA, double-antibody format with a biotin-labeled detection antibody and HRP-Streptavidin, TMB substrate development, and absorbance read at 450 nm. Validated recovery and linearity data are provided for serum, EDTA plasma, and heparin plasma samples. Reagents are stored at 2-8°C for 12 months, offering a robust tool for investigating NF-κB activity in basic and translational research.