Rat Nuclear Factor I/X (NFIX) ELISA Kit

Nuclear Factor I/X (NFIX) is a transcription factor of the Nuclear Factor I family with roles in brain development, skeletal muscle differentiation, hematopoiesis, and gene-expression programs active in chondrocyte and lung maturation. The Rat NFIX ELISA Kit from Assay Genie enables quantitative measurement of NFIX protein levels in rat-derived samples, supporting research into developmental biology, neuroscience, and disease-model characterization.

Each kit supports up to 96 individual sample determinations and uses an absorbance-based readout compatible with standard microplate readers. The assay is validated for tissue homogenates and similar rat sample preparations, and is used by investigators to follow up gene-expression studies at the protein level, validate knockdown or overexpression experiments, and profile NFIX abundance across tissues and experimental conditions.

The package contains a pre-coated microplate, matched antibody reagents, and protein standards required to build a standard curve. The workflow includes sample addition, incubation, washing, detection-antibody binding, and substrate development steps. Full procedural details, recommended dilutions, and performance characteristics are documented in the manual supplied with the kit.